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rabbit polyclonal anti chop gadd153  (Proteintech)


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    Proteintech rabbit polyclonal anti chop gadd153
    Rabbit Polyclonal Anti Chop Gadd153, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+chop+gadd153/CHOP%3B+GADD153+Fusion+Protein/pmc13002879-182-48-53
    Average 94 stars, based on 4 article reviews
    rabbit polyclonal anti chop gadd153 - by Bioz Stars, 2026-10
    94/100 stars

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    Western Blot:

    Article Title: The Impact of NRF2 Inhibition on Drug-Induced Colon Cancer Cell Death and p53 Activity: A Pilot Study
    Article Snippet: Enzymatic signal was visualized by chemiluminescence (ECL Detection system, Amersham GE Healthcare, Milan, Italy). .. The antibodies used for Western blotting analyses were: mouse monoclonal anti-HO-1, mouse monoclonal p53 (DO1), rabbit polyclonal phospho-Ser46, rabbit polyclonal p21, mouse monoclonal anti-PARP (poly ADP-ribose polymerase 1, cleaved form), mouse monoclonal anti-phospho-Histone H2AX (Ser139) (all from, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-NRF2 (Abcam, Cambridge, UK), rabbit polyclonal anti-CHOP (GADD153) (Proteintech, Rosemont, IL, USA), and mouse monoclonal β-actin (Calbiochem, San Diego, CA, USA). .. Densitometry was performed on ECL results with ImageJ software ( https://imagej.nih.gov , accessed on 10 February 2022) and relative band intensity normalized to β-actin and plotted as protein expression/β-actin ratio.

    Article Title: ATF6 prevents DNA damage and cell death in colon cancer cells undergoing ER stress
    Article Snippet: .. To evaluate protein expression on western blot membranes the primary antibody used were mouse monoclonal anti-p53 (clone DO-1) (sc-126, Santa Cruz Biotechnology), rabbit monoclonal anti-PARP (46D11) (9532; Cell Signaling, Danvers, MA, USA) rabbit polyclonal anti-BiP/GRP78 (11587-1-AP; Proteintech, Rosemont, IL, USA), rabbit monoclonal anti-CHOP (66741-1-Ig; Proteintech), mouse monoclonal anti-γH2AX (Ser 139) (sc-517348; Santa Cruz Biotechnology), mouse monoclonal anti-BRCA-1 (OP92; EMD Millipore, Burlington, MA, USA), rabbit polyclonal anti-CHOP (GADD153) (15204-1-AP; Proteintech), rabbit polyclonal anti-XBP1s (24868-1-AP; Proteintech), rabbit polyclonal anti-ATF6 (24169-1-AP; Proteintech), rabbit polyclonal anti-HSP90 (13171-1-AP; Proteintech), rabbit polyclonal anti-p-mTOR (Ser2448) (2971; Cell Signaling), mouse monoclonal anti-mTOR (66888-1-Ig; Proteintech), rabbit monoclonal anti-Phospho-4E-BP1 (Thr37/46) (236B4; Cell Signaling), mouse monoclonal anti-4E-BP1 (60246-1-Ig; Proteintech). .. Mouse monoclonal anti-β-actin (A5316; Sigma-Aldrich) was used as loading control.

    Article Title: Targeting c-Myc Unbalances UPR towards Cell Death and Impairs DDR in Lymphoma and Multiple Myeloma Cells
    Article Snippet: NT membranes were further washed in 1 X PBS-0.1% Tween20 and then developed using ECL Blotting Substrate (Advansta, San Jose, CA, USA). .. The antibodies used to identify specific proteins in Western blot are listed as follows: rabbit polyclonal anti-PARP (1:500) (Cell Signaling, Danvers, MA, USA, 9542), mouse monoclonal anti-caspase-3 (1:100) (clone E-8) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-7272), rabbit polyclonal anti-XBP1 (1:1000) (Novus Biologicals, Littleton, CO, NB100-80861), rabbit polyclonal anti-CHOP (GADD153) (1:1000) (Proteintech, Rosemont, IL, USA, 15204-1-AP), rabbit polyclonal anti-phospho-EIF2α (Ser51) (1:1000) (Cell Signaling, Danvers, MA, USA, 9721), rabbit polyclonal anti-EIF2α (1:4000) (Cell Signaling, Danvers, MA, USA, 9722), mouse monoclonal anti-γH2AX (phospho-Ser 139) (1:100) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-517348), mouse monoclonal anti-BRCA1 (1:1000) (EMD Millipore, Burlington, MA, OP92), mouse monoclonal anti-RAD51 (1:200) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-377467), rabbit polyclonal anti-c-MYC (1:500) (Proteintech, Rosemont, IL, USA, 10828-1-AP). .. Mouse monoclonal anti-β Actin (1:10,000) (Sigma-Aldrich, Burlington, MA, USA) was used as loading control.

    Article Title: Zinc Supplementation Enhances the Pro-Death Function of UPR in Lymphoma Cells Exposed to Radiation
    Article Snippet: .. The following primary antibodies were used for Western blot analysis: mouse monoclonal anti-ATF4 (NovusBIO, CO, USA, cat# MAB7218) 1:3000, rabbit polyclonal anti-BiP/GRIP78 (Proteintech, Rosemont, IL, USA, cat# 11587-1-AP) 1:5000, rabbit polyclonal anti-CHOP (GADD153) (Proteintech, cat# 15204-1-AP) 1:1000, mouse monoclonal anti-DUSP5 (Santa Cruz Biotechnology Inc., Dallas, TX, USA cat# sc-393801) 1:200, rabbit polyclonal anti-ERK1 (Santa Cruz Biotechnology Inc. cat# sc-93) 1:200, rabbit polyclonal anti-ERK2 (Santa Cruz Biotechnology Inc. cat# sc-154) 1:200, mouse monoclonal anti-p-ERK (Santa Cruz Biotechnology Inc. cat# sc-7383) 1:500, mouse monoclonal anti -pH2AX (Ser 139) (Santa Cruz Biotechnology Inc. cat# sc-517348) 1:100, rabbit polyclonal anti-PARP1 (Proteintech, cat# 13371-1) 1:1000, rabbit polyclonal anti -p21 (clone C-19, Santa Cruz Biotechnology Inc. cat# sc-397) 1:200, mouse monoclonal anti -p53 (clone DO-1, Santa Cruz Biotechnology Inc. cat# sc-126) 1:100 and Mouse monoclonal anti -β-actin (Sigma Aldrich cat# A5441) 1:10,000 was used as loading control. .. Goat anti-rabbit IgG-horseradish peroxidase HRP (Santa Cruz Biotechnology, Inc. Heidelberg, Germany, cat# sc-2004) 1:10,000, goat anti-mouse IgG-horseradish peroxidase HRP (Santa Cruz Biotechnology, Inc. Heidelberg, Germany, cat#sc-2005) 1:10,000 were used as secondary antibodies.

    Article Title: Role of UPR Sensor Activation in Cell Death–Survival Decision of Colon Cancer Cells Stressed by DPE Treatment
    Article Snippet: The gels were transferred to nitrocellulose membranes (Biorad, Hercules) for 1.5 h in Tris–glycine buffer and the membranes were blocked in 1× PBS-0.1% Tween20 solution containing 3% of BSA (Serva), probed with specific antibodies, and developed using ECL Blotting Substrate (Advansta). .. To evaluate protein expression on Western blot membranes, the following antibody were used: rabbit polyclonal anti-ATF6 (1:600) (Proteintech, 24169-1-AP), rabbit polyclonal anti-XBP1 (1:1000) (Novus Biologicals, NB100-80861), rabbit polyclonal anti-phospho-eIF2alpha (Ser51) (1:1000) (Cell Signaling, 9721), rabbit polyclonal anti-eIF2alpha (1:4000) (Cell Signaling, 9722), rabbit polyclonal anti-BiP/GRIP78 (1:5000) (Proteintech, 11587-1-AP), rabbit polyclonal anti-CHOP (GADD153) (1:1000) (Proteintech, 15204-1-AP), mouse monoclonal anti-DUSP5 (1:200) (Santa Cruz Biotechnology Inc., Dallas, TX, USA sc-393801), mouse monoclonal anti-p-ERK (Santa Cruz Biotechnology Inc., sc-7383), rabbit polyclonal anti-ERK1 (Santa Cruz Biotechnology Inc., sc-93), rabbit polyclonal anti-ERK2 (Santa Cruz Biotechnology Inc., sc-154), rabbit monoclonal anti-Mcl1 (1:1000) (Cell Signaling, 39224), mouse monoclonal anti-p53 (1:100) (clone DO-1, Santa Cruz Biotechnology Inc., sc-126). .. Mouse monoclonal anti-β-actin (1:10,000) (Sigma Aldrich) was used as the loading control.

    Article Title: NRF2 and Bip Interconnection Mediates Resistance to the Organometallic Ruthenium-Cymene Bisdemethoxycurcumin Complex Cytotoxicity in Colon Cancer Cells.
    Article Snippet: .. To detect the protein expression on Western blot membranes, the following antibodies were used: mouse monoclonal anti-p62/SQSTM1 (D-3, sc-28359) (1:1000), mouse monoclonal anti-HO-1 (A-3, sc-136960) (1:1000) and mouse monoclonal anti-NQO1/A180, sc-32793) (Santa Cruz Biotechnology Inc, Dallas, TX, USA), rabbit polyclonal anti-NRF2 (1:1000) (Abcam, Cambridge, UK, #ab62352), mouse monoclonal anti-phospho-Histone H2AX (Ser139 clone JBW301) (1:1000) (Sigma-Aldrich, St Louis, MO, USA, #05-636), rabbit polyclonal anti-CHOP (GADD153) (1:1000) (Proteintech, Rosemont, IL; USA, #15204-1-AP), and rabbit polyclonal anti-BiP/GRP78 (1:5000) (Proteintech,Rosemont, USA, #11587-1-AP). .. Mouse monoclonal β-actin (Ab-1) (1:10,000) (Calbiochem, San Diego, CA, USA, #CP01), was used as protein loading control.

    Article Title: Inhibiting HSP27 activates the XBP1s/CerS1 interplay, which triggers DRP1-driven mitophagy, thereby protecting against cell death and promoting the KSHV lytic cycle in primary effusion lymphoma cells
    Article Snippet: Blots were subsequently washed 3X with PBS-0,1% Tween and incubated with the appropriate secondary antibody HRP-conjugated at a dilution of 1:10000 for 30 minutes at room temperature, Membranes were finally subjected to ECL (Advansta, San Jose, CA, USA, cat n. 12045-D20). .. The following primary antibodies were used in western blots: rabbit polyclonal anti-PARP (1:1000) (Cell Signaling, Danvers, MA, USA, cat n. 9542), mouse monoclonal anti-CerS1 (LASS1) (1:500) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, cat. n. 293497), rabbit polyclonal anti-BiP/GRIP78 (1:5000) (; Proteintech, Rosemont, IL, USA, cat n. 11587-1-AP), rabbit polyclonal anti-CHOP (GADD153) (1:1000) (Proteintech, Rosemont, IL, USA, cat n. 15204-1-AP), rabbit polyclonal anti-XBP1 (1:500) (Novus Biologicals, Centennial, CO, USA; cat n. NBP1-77681SS), mouse monoclonal anti-DRP1 (1:500) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, cat. n. sc-271583), mouse monoclonal anti-HADHA (1:500) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, cat. n. sc-374497), mouse monoclonal anti-Parkin (1:500) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, cat. n. sc-32283), rabbit polyclonal anti-LC3 I/II (1:1000) (Novus Biologicals, Centennial, CO, USA cat. n. NB100–2220), rabbit polyclonal anti-SQSTM1/p62 (1:500) (Novus Biologicals, Centennial, CO, USA cat. n. NBP-48-320), mouse monoclonal anti-HSP27 (1:1000) (Proteintech, Rosemont, IL, USA, cat n. 182841), rabbit polyclonal anti-HSP110 (1:200) (Abcam, Cambridge UK, cat. n. ab24503). .. The following antibodies were used for loading controls: mouse monoclonal anti-GAPDH (1:10,000) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, cat n. 47724), rabbit polyclonal anti-Histone H3 (1:500) (Cell Signalling Danvers, MA, USA, cat n. 9715).

    Article Title: NRF2 and Bip Interconnection Mediates Resistance to the Organometallic Ruthenium-Cymene Bisdemethoxycurcumin Complex Cytotoxicity in Colon Cancer Cells
    Article Snippet: Densitometry was performed on ECL results with ImageJ software (1.47 version, NIH, Bethesda, MD, USA) which was downloaded from the NIH website ( http://imagej.nih.gov/ij , accessed on 1 August 2022) and the relative band intensity was normalized to β-actin signals and plotted as protein expression/β-actin ratio. .. To detect the protein expression on Western blot membranes, the following antibodies were used: mouse monoclonal anti-p62/SQSTM1 (D-3, sc-28359) (1:1000), mouse monoclonal anti-HO-1 (A-3, sc-136960) (1:1000) and mouse monoclonal anti-NQO1/A180, sc-32793) (Santa Cruz Biotechnology Inc, Dallas, TX, USA), rabbit polyclonal anti-NRF2 (1:1000) (Abcam, Cambridge, UK, #ab62352), mouse monoclonal anti-phospho-Histone H2AX (Ser139 clone JBW301) (1:1000) (Sigma-Aldrich, St Louis, MO, USA, #05-636), rabbit polyclonal anti-CHOP (GADD153) (1:1000) (Proteintech, Rosemont, IL; USA, #15204-1-AP), and rabbit polyclonal anti-BiP/GRP78 (1:5000) (Proteintech,Rosemont, USA, #11587-1-AP). .. Mouse monoclonal β-actin (Ab-1) (1:10,000) (Calbiochem, San Diego, CA, USA, #CP01), was used as protein loading control.

    Expressing:

    Article Title: ATF6 prevents DNA damage and cell death in colon cancer cells undergoing ER stress
    Article Snippet: .. To evaluate protein expression on western blot membranes the primary antibody used were mouse monoclonal anti-p53 (clone DO-1) (sc-126, Santa Cruz Biotechnology), rabbit monoclonal anti-PARP (46D11) (9532; Cell Signaling, Danvers, MA, USA) rabbit polyclonal anti-BiP/GRP78 (11587-1-AP; Proteintech, Rosemont, IL, USA), rabbit monoclonal anti-CHOP (66741-1-Ig; Proteintech), mouse monoclonal anti-γH2AX (Ser 139) (sc-517348; Santa Cruz Biotechnology), mouse monoclonal anti-BRCA-1 (OP92; EMD Millipore, Burlington, MA, USA), rabbit polyclonal anti-CHOP (GADD153) (15204-1-AP; Proteintech), rabbit polyclonal anti-XBP1s (24868-1-AP; Proteintech), rabbit polyclonal anti-ATF6 (24169-1-AP; Proteintech), rabbit polyclonal anti-HSP90 (13171-1-AP; Proteintech), rabbit polyclonal anti-p-mTOR (Ser2448) (2971; Cell Signaling), mouse monoclonal anti-mTOR (66888-1-Ig; Proteintech), rabbit monoclonal anti-Phospho-4E-BP1 (Thr37/46) (236B4; Cell Signaling), mouse monoclonal anti-4E-BP1 (60246-1-Ig; Proteintech). .. Mouse monoclonal anti-β-actin (A5316; Sigma-Aldrich) was used as loading control.

    Article Title: Role of UPR Sensor Activation in Cell Death–Survival Decision of Colon Cancer Cells Stressed by DPE Treatment
    Article Snippet: The gels were transferred to nitrocellulose membranes (Biorad, Hercules) for 1.5 h in Tris–glycine buffer and the membranes were blocked in 1× PBS-0.1% Tween20 solution containing 3% of BSA (Serva), probed with specific antibodies, and developed using ECL Blotting Substrate (Advansta). .. To evaluate protein expression on Western blot membranes, the following antibody were used: rabbit polyclonal anti-ATF6 (1:600) (Proteintech, 24169-1-AP), rabbit polyclonal anti-XBP1 (1:1000) (Novus Biologicals, NB100-80861), rabbit polyclonal anti-phospho-eIF2alpha (Ser51) (1:1000) (Cell Signaling, 9721), rabbit polyclonal anti-eIF2alpha (1:4000) (Cell Signaling, 9722), rabbit polyclonal anti-BiP/GRIP78 (1:5000) (Proteintech, 11587-1-AP), rabbit polyclonal anti-CHOP (GADD153) (1:1000) (Proteintech, 15204-1-AP), mouse monoclonal anti-DUSP5 (1:200) (Santa Cruz Biotechnology Inc., Dallas, TX, USA sc-393801), mouse monoclonal anti-p-ERK (Santa Cruz Biotechnology Inc., sc-7383), rabbit polyclonal anti-ERK1 (Santa Cruz Biotechnology Inc., sc-93), rabbit polyclonal anti-ERK2 (Santa Cruz Biotechnology Inc., sc-154), rabbit monoclonal anti-Mcl1 (1:1000) (Cell Signaling, 39224), mouse monoclonal anti-p53 (1:100) (clone DO-1, Santa Cruz Biotechnology Inc., sc-126). .. Mouse monoclonal anti-β-actin (1:10,000) (Sigma Aldrich) was used as the loading control.

    Article Title: NRF2 and Bip Interconnection Mediates Resistance to the Organometallic Ruthenium-Cymene Bisdemethoxycurcumin Complex Cytotoxicity in Colon Cancer Cells.
    Article Snippet: .. To detect the protein expression on Western blot membranes, the following antibodies were used: mouse monoclonal anti-p62/SQSTM1 (D-3, sc-28359) (1:1000), mouse monoclonal anti-HO-1 (A-3, sc-136960) (1:1000) and mouse monoclonal anti-NQO1/A180, sc-32793) (Santa Cruz Biotechnology Inc, Dallas, TX, USA), rabbit polyclonal anti-NRF2 (1:1000) (Abcam, Cambridge, UK, #ab62352), mouse monoclonal anti-phospho-Histone H2AX (Ser139 clone JBW301) (1:1000) (Sigma-Aldrich, St Louis, MO, USA, #05-636), rabbit polyclonal anti-CHOP (GADD153) (1:1000) (Proteintech, Rosemont, IL; USA, #15204-1-AP), and rabbit polyclonal anti-BiP/GRP78 (1:5000) (Proteintech,Rosemont, USA, #11587-1-AP). .. Mouse monoclonal β-actin (Ab-1) (1:10,000) (Calbiochem, San Diego, CA, USA, #CP01), was used as protein loading control.

    Article Title: NRF2 and Bip Interconnection Mediates Resistance to the Organometallic Ruthenium-Cymene Bisdemethoxycurcumin Complex Cytotoxicity in Colon Cancer Cells
    Article Snippet: Densitometry was performed on ECL results with ImageJ software (1.47 version, NIH, Bethesda, MD, USA) which was downloaded from the NIH website ( http://imagej.nih.gov/ij , accessed on 1 August 2022) and the relative band intensity was normalized to β-actin signals and plotted as protein expression/β-actin ratio. .. To detect the protein expression on Western blot membranes, the following antibodies were used: mouse monoclonal anti-p62/SQSTM1 (D-3, sc-28359) (1:1000), mouse monoclonal anti-HO-1 (A-3, sc-136960) (1:1000) and mouse monoclonal anti-NQO1/A180, sc-32793) (Santa Cruz Biotechnology Inc, Dallas, TX, USA), rabbit polyclonal anti-NRF2 (1:1000) (Abcam, Cambridge, UK, #ab62352), mouse monoclonal anti-phospho-Histone H2AX (Ser139 clone JBW301) (1:1000) (Sigma-Aldrich, St Louis, MO, USA, #05-636), rabbit polyclonal anti-CHOP (GADD153) (1:1000) (Proteintech, Rosemont, IL; USA, #15204-1-AP), and rabbit polyclonal anti-BiP/GRP78 (1:5000) (Proteintech,Rosemont, USA, #11587-1-AP). .. Mouse monoclonal β-actin (Ab-1) (1:10,000) (Calbiochem, San Diego, CA, USA, #CP01), was used as protein loading control.

    Control:

    Article Title: Zinc Supplementation Enhances the Pro-Death Function of UPR in Lymphoma Cells Exposed to Radiation
    Article Snippet: .. The following primary antibodies were used for Western blot analysis: mouse monoclonal anti-ATF4 (NovusBIO, CO, USA, cat# MAB7218) 1:3000, rabbit polyclonal anti-BiP/GRIP78 (Proteintech, Rosemont, IL, USA, cat# 11587-1-AP) 1:5000, rabbit polyclonal anti-CHOP (GADD153) (Proteintech, cat# 15204-1-AP) 1:1000, mouse monoclonal anti-DUSP5 (Santa Cruz Biotechnology Inc., Dallas, TX, USA cat# sc-393801) 1:200, rabbit polyclonal anti-ERK1 (Santa Cruz Biotechnology Inc. cat# sc-93) 1:200, rabbit polyclonal anti-ERK2 (Santa Cruz Biotechnology Inc. cat# sc-154) 1:200, mouse monoclonal anti-p-ERK (Santa Cruz Biotechnology Inc. cat# sc-7383) 1:500, mouse monoclonal anti -pH2AX (Ser 139) (Santa Cruz Biotechnology Inc. cat# sc-517348) 1:100, rabbit polyclonal anti-PARP1 (Proteintech, cat# 13371-1) 1:1000, rabbit polyclonal anti -p21 (clone C-19, Santa Cruz Biotechnology Inc. cat# sc-397) 1:200, mouse monoclonal anti -p53 (clone DO-1, Santa Cruz Biotechnology Inc. cat# sc-126) 1:100 and Mouse monoclonal anti -β-actin (Sigma Aldrich cat# A5441) 1:10,000 was used as loading control. .. Goat anti-rabbit IgG-horseradish peroxidase HRP (Santa Cruz Biotechnology, Inc. Heidelberg, Germany, cat# sc-2004) 1:10,000, goat anti-mouse IgG-horseradish peroxidase HRP (Santa Cruz Biotechnology, Inc. Heidelberg, Germany, cat#sc-2005) 1:10,000 were used as secondary antibodies.



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